The Effect of Adding Protein-Xanthophylls Concentrate (PX) from Lucerne (Medicago sativa) on Growth Parameters and Redox Profile in Muscles of carp, Cyprinus carpio (L.)

The effect of adding protein xanthophyll from lucerne (PX) on growth parameters and the redox profile of carp muscle Cyprinus carpio (L.) was studied. During a 10-week experiment, carp were kept in two types of tanks: Control, where fish were fed a commercial fish feed, and in experimental tanks (PX+), where the fish were fed the same fish feed with a 5% addition protein xanthophyll from lucerne. Weekly for all fish the total length (Tl), standard length (Sl) and body weight (W) were measured. After experiment the coefficient of condition, specific growth rate (SGR) was calculated and with the spectrophotometric method oxidative status (MDA, SOD, Vit. C, CAT i H2O2) in carp muscle, was estimated. The results of observations have shown that the addition of 5% protein-xanthophyll concentrate (PX) from lucerne, to feed fish resulted in a greater increase (P>0.05) of total length and body weight of carp. Moreover, the addition of lucerne caused slightly better coefficients of condition and SGR. The addition of lucerne to fish feed affected the higher values of MDA (P<0.0001), SOD and CAT in the muscle of carp, at the same time the fish had a slightly lower value for content of Vit. C (P<0.0002) and H2O2. The addition of PX to carp feed induced an increase in products of the peroxidation lipid with simultaneous stimulation of the antioxidant enzymatic system in carp muscle.